Antibiotic G-418 is an aminoglycoside antibiotic similar in structure to Gentamicin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. It is also known as geneticin, a selective antibiotic.
The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5µg/ml or less are recommended. In general, concentrations of approximately 400 µg/ml for selection and 200 µg/ml for maintenance are required for mammalian cells.