The most common pyrogens are endotoxins which are lipopolysaccharides (LPS) produced by Gram-negative bacteria such as E. coli. The presence of these molecules in cell culture reagents, as well as media or buffer, can modify cell biological effect/answer, in particular in immunology aera. Using pyrogen-free water in cell application avoids the endotoxin’s impact.
Biowest offers Cell Culture Water, a pyrogen-free water, who is suitable to dissolve dry powder cell culture media or prepare all cell culture reagents needed.