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Diamond as Functional Material for Bioelectronics and Biotechnology
Authors:Bohuslav Rezek, Marie Krátká, Egor Ukraintsev, Oleg Babchenko, Alexander Kromka, Antonín Brož and Marie Kalbacova Read the publication in PDF
Auto-Luminescent Genetically-Encoded Ratiometric Indicator for Real-Time Ca2+ Imaging at the Single Cell Level
Authors Kenta Saito, Noriyuki Hatsugai, Kazuki Horikawa, Kentaro Kobayashi, Toru Matsu-ura, Katsuhiko Mikoshiba, Takeharu Nagai BackgroundEfficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near real time without external light illumination. Methodology/Principal…
Licochalcone A Potently Inhibits Tumor Necrosis Factor α-Induced Nuclear Factor-κB Activation through the Direct Inhibition of IκB Kinase Complex Activation
Authors:Megumi Funakoshi-Tago, Saeko Tanabe, Kenji Tago, Hiroshi Itoh, Tadahiko Mashino, Yoshiko Sonoda and Tadashi Kasahara Glycyrrhiza inflata has been used as a traditional medicine with anti-inflammatory activity; however, its mechanism has not been fully understood. Licochalcone A is a major and biogenetically characteristic chalcone isolated from G. inflata. Here, we found that licochalcone A…
Long-term Cultures of Bone Marrow–Derived Human Mesenchymal Stem Cells Frequently Undergo Spontaneous Malignant Transformation
Authors:Gro Vatne Røsland, Agnete Svendsen, Anja Torsvik, Ewa Sobala, Emmet McCormack, Heike Immervoll, Josef Mysliwietz, Joerg-Christian Tonn, Roland Goldbrunner, Per Eystein Lønning, Rolf Bjerkvig, and Christian Schichor Human mesenchymal stem cells (hMSC) aid in tissue maintenance and repair by differentiating into specialized cell types. Due to this ability, hMSC are currently being evaluated for…
Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months
Authors:Taro Ishigaki, Kazuhiro Sudo, Takashi Hiroyama, Kenichi Miharada, Haruhiko Ninomiya, Shigeru Chiba, Toshiro Nagasawa, and Yukio Nakamura We previously reported that long-lasting in vitro hematopoiesis could be achieved using the cells differentiated from primate embryonic stem (ES) cells. Thus, we speculated that hematopoietic stem cells differentiated from ES cells could sustain long-lasting in vitro…
Cholesterol Biosynthesis Pathway Intermediates and Inhibitors Regulate Glucose-Stimulated Insulin Secretion and Secretory Granule Formation in Pancreatic β-Cells
Authors:Miho Tsuchiya, Masahiro Hosaka, Tomohisa Moriguchi, Shaojuan Zhang, Masayuki Suda, Hiromi Yokota-Hashimoto, Kazuo Shinozuka and Toshiyuki Takeuchi Cholesterol is reportedly abundant in the endocrine secretory granule (SG) membrane. In this study, we examined the involvement of cholesterol biosynthesis intermediates and inhibitors in insulin secretion and SG formation mechanisms. There are two routes for the…